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Titanium Dioxide Nanoparticles d its Impact on Growth Biomas

Anthropogenic activities of developing and developed countries caused environmental pollution. Environmental pollution causes stress to plants and agricultural yield loss and also imbalance the global food security. Titanium dioxide (TiO) is considered as a beneficial element for plant growth and development and also one of the most widely used in the consumer products, agriculture and energy sectors. Titanium dioxide (TiO) applied via roots or leaves at low concentrations has been documented to improve crop performance through stimulating the activity of certain enzymes, enhancing chlorophyll content and photosynthesis, promoting nutrient uptake, strengthening stress tolerance and improving crop yield and quality. Widespread application ofnanoparticles caused damage to organisms and ecosystems. For a better understanding of TiOnanoparticle toxicity in living organisms may promote risk assessment and safe use practices of these nanomaterials. This review summarizes the beneficial effect of TiOnanoparticle on plant growth and development and its protective role against abiotic and biotic stress.

IndraJeet Chaudhary and Vivek Singh, 2020. Titanium Dioxide Nanoparticles and its Impact on Growth, Biomass and Yield of Agricultural Crops under Environmental Stress: A Review.Research Journal of Nanoscience and Nanotechnology, 10: 1-8.

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Environmental pollution is a burning problem caused biotic and abiotic stresses to plants and yield loss of crops1. The exponential increase in the worlds population leads to an increased demand for food. Agricultural production and productivity are directly linked with nutrient availability. For sustained high crop yields, the application of fertilizer efficiently required for the farmer for crop production. Therefore, the productivity of agricultural crops depends on the application of fertilizer23. In this contest for maintenance of crop production and nutrient management is the most important way of sustainable agriculture. Titanium dioxide nanoparticle can be synthesized as a natural way and widely used in commercial products such as sunscreens and toothpaste, industrial products like paints, lacquers and paper and in photocatalytic processes such as water treatment47. The annual production oftitanium dioxidenanoparticles (TiO2) was more than 10,000 metric t8. The mass production, extensive use and uncontrolled disposal of TiO2nanoparticle will inevitably lead to their release into the environment, where they may exert harm to individual organisms and ecosystems910.

The toxic effects of TiO2NPs on plants11and animal cells12have been demonstrated in multiple studies. Therefore, understanding the TiO2nanoparticle-induced effects on individual organisms and the mechanisms of their action is of great importance in assessing environmental risk. The literature on TiO2nanoparticle effects in plants is limited. Toxic effects of TiO2nanoparticle with a 100-nm diameter have been observed in the higher plantsAllium cepaandNicotiana tabacum. Nano-size of TiO2inducedgrowth inhibition, DNA damage andlipid peroxidationinAllium ceparoot and also DNA damage in theNicotiana tabacumleaf13. Investigations into the toxicity of TiO2nanoparticle to plants mainly focused on plant growth and genotoxicity1415. ROS andfree radicals directly destroy the chlorophyll molecule, cause membranelipid peroxidationand degradation of chlorophyll, protein,relative water contents resulting in the aggravation of membrane damage16. In addition, ROS can attack theamino acidresidues of proteins to form carbonyl derivatives. H2O2can produce more active OHthrough the Haber-Weiss reaction, leading to membrane lipid per-oxidation, base mutation, DNA strand breaks and protein damage. Although, these studies have substantially increased our knowledge about TiO2nanoparticles toxicity to plants.

Titanium dioxide (TiO2) nanoparticles are also used as an essential nutrient for plant growth and development1718and also improves the photosynthetic pigments in plants such as chlorophyll (a and b), carotenoids and anthocyanins contents, therefore, enhanced the crop growth and yield19. Various researchers reported that thetitanium dioxidenanoparticle caused positive effects on plant growth i.e., canola (Brassica napus)20,Solanum lycopersicum(L.) andVigna radiata(L.)21. While the study by Da Costa and Sharma22found phytotoxicity oftitanium dioxidenanoparticle onOryza sativawhich was observed through a decrease in seedling growth, photosynthetic activity and biochemical processes at their elevated concentration (1000 ppm). Tripathiet al.23elaborately reviewed the number of phytotoxicity created by manufactured nanoparticles indifferent plants on the ground of physiological, biochemical, genetic and molecular levels. The effect oftitanium dioxidenanoparticle on metabolic processes has been studied and was found to affect processes such as hormone metabolism24. However, the effects oftitanium dioxidenanoparticles are still unclear. In the present review, discussion was carried out on the current developments in plant science that attention on the role of nanoparticles (NPs) in plant growth and development and also on plant stress tolerance mechanisms.

APPLICATIONS OF TIO2NANOPARTICLES IN AGRICULTURAL CROPS

Nanotechnology is not a single technology but it is a mixture of several technological groups, which is working at the nano-level25. In the field of agriculture, nanotechnology has huge potential applications, for instance, in modern agricultural systems nanotechnology ease of chemical release and also use of nano-sensors for recording the data of environmental stress, crop setting, resistance against the biotic and abiotic factors via improving the quality of plants2527.

According to Parisiet al.28, European commission assessed that, mainly in developed countries, the main use of nanotechnology is pesticide formulations and nanosensors, which are very helpful in applied and sustainable agricultural practices. Nanotechnology also provides the various biotic and abiotic stresses in way of plant life. Various beneficial and harmful influences of nanoparticles were found out by different research workers in the field of agriculture, Fracetoet al.17observed in some research works that mainly focus on the agriculturally positive side of nanoparticles.

TiO2(titanium oxide) nanoparticles by increasing essential metal nutrient uptake reported to enhances plant development1718. In the support of the above statement, Mortezaet al.19suggested thattitanium dioxidenanoparticle enhanced crop yield by improving the chlorophyll (a and b), carotenoids and anthocyanins contents with their concentration of 0.01 and 0.03%, respectively. Similar studies were elaborated with positive effects oftitanium dioxidenanoparticle in canola (Brassica napus)29,Solanum lycopersicum(L.) andVigna radiata(L.)21. While a study by Da Costa and Sharma22found phytotoxicity oftitanium dioxidenanoparticle onOryza sativawhich was observed through a decrease in seedling growth, photosynthetic activity and biochemical processes at their elevated concentration (1000 ppm). Tripathiet al.23decoratively reviewed the number of phytotoxicity created by industrial nanoparticles indiverse plants on the ground of physiological, biochemical, genetic and molecular.

IMPACT OF TITANIUM DIOXIDE NANOPARTICLES ON AGRICULTURAL PLANTS

The efficiency of nanoparticle is basically justified by theirchemical compositionlike, size of particles, surface cover area, reactive abilities with effective doses30. Titanium dioxide nanoparticles play an important role in plant growth and development of plants. When nanoparticles link with plant showing several changes in their morphology and physiology that sole depending on nature and properties of nanoparticles. The different research results showed that TDN responds to both positive and negative ways of plant growth and development31. The influence of nanoparticle concentration on various species is highly variable. That way, this particular review covers the various aspects of nanoparticle in seed germination, root growth, root and shoot biomass with photosynthesis rate.

Titanium dioxide nanoparticle enhanced seed germination and promoted radicle and growth of canola seedlings29. Jaberzadehetal.32reported thattitanium dioxidenanoparticle augmented wheat plant growth andyield componentsunderwater deficitstress conditions. Titanium dioxide nanoparticle regulates enzymes activity involved in nitrogen metabolisms such as nitrate reductase, glutamate dehydrogenase, glutamine synthase and glutamic-pyruvic transaminase that helps the plants to absorb nitrate and also special treatment the conversion of inorganic nitrogen to organic nitrogen in the form of protein and chlorophyll, that could increase the plant biomass3334. It also acts as a photocatalyst and induces an oxidation-reduction reaction35. Therefore promote the chlorophyll formation and stimulate Ribulose 1, 5-bisphosphate carboxylase (Rubisco) activity and increases photosynthesis, thereby increasing plant growth and development33. Titanium dioxide nanoparticle increases light absorbance, hasten the transport and conversion of the light energy, protect chloroplasts from aging and prolong the photosynthetic time of the chloroplasts33. It may be due totitanium dioxidenanoparticle protects the chloroplast from excessive light by augmenting the activity of antioxidant enzymes, such as catalase, peroxidase,superoxide dismutaseFig. 1). Overalltitanium dioxidenanoparticle enhanced growth biomass and yield of crop cultivars by promoting nutrient uptake efficiency and increasing photosynthesis rate of plants.

Impact on quantitative and qualitative traits of agricultural plants:Titanium (Ti) is considered a beneficial element for plant growth and development. Application of Ti via roots or leaves at low concentrations has been documented to improve crop performance through stimulating the activity of certain enzymes, enhancing chlorophyll content and photosynthesis, promoting nutrient mobilization, biotic and abiotic stress tolerances and improving crop yield and quality. There has been an increasing amount of attention in the literature regarding the effects oftitanium dioxidenanoparticle on plant performance (Table 1). Titanium dioxide nanoparticles suspensions have been used to study the impact on seed germination of various crops. The result showed thattitanium dioxidenanoparticles increased the germination rate, root length with improved growth rate of various varieties i.e.,Arabidopsis thaliana(L.), cabbage, corn, oilseed rape, canola, cucumber, fennel, lettuce, oat, onion, parsley, red clover, soybean, spinach, tomato and wheat83644.

Effect oftitanium dioxidenanoparticle against plant stress:Now, these days environmental pollution is a problem worldwide and caused a negative impact on vegetation and plants. Biotic and abiotic stresses negatively affected the plant growth and biomass also reduced the yield of cultivars14546. Titanium dioxide increased plant tolerance to abiotic and biotic stress (Table 2). Various study reported about application oftitanium dioxideincreased cold stress tolerance in chickpea (Cicer arietinumL.)2947,heat stressin tomato48, drought in wheat32and flax (Linum usitatissiumL.)49, cadmium toxicity in green algae (Chlamydomonas reinhardtiiP.A. Dang) and soybean5021and bacterial spot disease caused by Xanthomonas perforans in tomato51. Foliar spray oftitanium dioxidenanoparticle increased chlorophyll content in tomato and oilseeds52, enhanced the activity of Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) and promoted net photosynthesis in Arabidopsis53, spinach5457, tomato48and basil (Ocimum basilicumL.)58. Titanium dioxide nanoparticle treatments significantly increased crop yield or biomass of barley59, corn6019, mung bean (Vigna radiateL.), snail clover (Medicago scutellataMil.), tomato6162and wheat63.

Nano-anatase TiO2has a photocatalyzed characteristic and improves the light absorbance and the transformation from light energy to electrical and chemical energy and also induces carbon dioxide assimilation and protects chloroplast from aging for long time illumination335455. Nano-anatasetitanium dioxidenanoparticle enhances the photosynthetic carbon assimilation by activating Rubisco (a complex of Rubisco and Rubisco activase) that could promote Rubisco carboxylation, thereby increasing the growth of plants64. Maetal.65studied the impact of nano-anatase on the molecular mechanism of carbon reaction and suggested a nano-anatase-induced marker gene for Rubisco activase (RCA) mRNA and enhanced protein levels and activities of Rubisco activase resulted in the improvement of the Rubisco carboxylation and the high rate of photosynthetic carbon reaction. The exogenous application oftitanium dioxidenanoparticle improves net photosynthetic rate, conductance to water and transpiration rate in plants48. According to Leiet al.57, nano-anatase promoted strongly whole chain electron transport, photoreduction activity of photosystem II, O2-evolving and photophosphorylation activity of chlorophyll under both visible and ultraviolet light.

DISADVANTAGES OF TITANIUM DIOXIDE NANOPARTICLE

Stresses induce crop loss and create food crises worldwide. The use oftitanium dioxidenanoparticles may not show only the right direction impact but also caused a negative impact on plant growth. As shown inTable 3, but some results were a neutral or negative direction. The minimum positive influences oftitanium dioxidenanoparticles could be due to various key factors like the variation in species of plant, the physiological position of species at the time being measured, quality of seeds, sizes oftitanium dioxidenanoparticles with their consistency, objectives and experimental methods. In some trialstitanium dioxidenanoparticles is used at a concentration up to 5000 g mL1, normally such type of high volume of concentration may not found naturally in the normal environmental conditions, observations of the result could not provide complete information about the impact oftitanium dioxidenanoparticles in the plant body. While serious attention does need to be given to the necessity and outcome of usedtitanium dioxidenanoparticles within the environment and food chain6623, more detailed and specified research in this respect should be followed.

Due to the unique properties oftitanium dioxidenanoparticles, a handful of research work has been done on toxicological effects on plants, yet research converging on the understanding of the valuable effects of nanoparticle on plant remains incomplete. Some research works revealed thattitanium dioxidenanoparticles showed a constructive impact against stress by enhancing the growth and development of plants. Also, more studies are needed to see the sights mode of action of nanoparticle, their interface with biomolecules and their impact on the regulation ofgene expressions in plants.

This study discovers the impact oftitanium dioxidenanoparticles on plants that can be beneficial for protection against stress. This study will help the researcher to uncover the critical areas of agricultural production.

2:Chaudhary, I.J. and R.P. Singh, 2018.Triticum aestivumL. PBW-343) applied with organic matrix based slow release bio fertilizers. Int. J. Curr. Microbiol. Applied Sci., Special Issue-7: 3221-3238.

3:Shah, K.N., I.J. Chaudhary, D.K. Rana and V. Singh, 2019.Allium cepaL.) cultivar. Asian J. Agric. Res., 13: 20-27.

4:Keller, A.A., S. McFerran, A. Lazareva and S. Suh, 2013.

5:Wang, L., J. Li, Q. Zhou, G. Yang and X.L. Dinget al., 2014.

6:Mitrano, D.M., S. Motellier, S. Clavaguera and B. Nowack, 2015.

7:Wen, J., X. Li, W. Liu, Y. Fang, J. Xie and Y. Xu, 2015.2nanomaterials. Chin. J. Catal., 36: 2049-2070.

8:Szymaska, R., K. Kołodziej, I. Ślesak, P. Zimak-Piekarczyk and A. Orzechowskaet al., 2016.Arabidopsis thaliana. Environ. Pollut., 213: 957-965.

9:Khosravi-Katuli, K., E. Prato, G. Lofrano, M. Guida, G. Vale and G. Libralato, 2017.

10:Minetto, D., A.V. Ghirardini and G. Libralato, 2016.2, ZnO and C60engineered nanoparticles: An overview. Environ. Int., 92: 189-201.

11:Atha, D.H., H. Wang, E.J. Petersen, D. Cleveland and R.D. Holbrooket al., 2012.

12:Karlsson, H.L., P. Cronholm, J. Gustafsson asd L. Moller, 2008.

13:Ghosh, M., M. Bandyopadhyay and A. Mukherjee, 2010.2) nanoparticles at two trophic levels: Plant and human lymphocytes. Chemosphere, 81: 1253-1262.

14:Aslani, F., S. Bagheri, N.M. Julkapli, A.S. Juraimi, F.S.G. Hashemi and A. Baghdadi, 2014.

15:Chichiricc, G. and A. Poma, 2015.

16:Metzler, D.M., M. Li, A. Erdem and C.P. Huang, 2011.2particles with an emphasis on the effect of particle size. Chem. Eng. J., 170: 538-546.

17:Fraceto, L.F., R. Grillo, G.A. de Medeiros, V. Scognamiglio, G. Rea and C. Bartolucci, 2016.

18:Khot, L.R., S. Sankaran, J.M. Maja, R. Ehsani and E.W. Schuster, 2012.

19:Morteza, E., P. Moaveni, H.A. Farahani and M. Kiyani, 2013.Zea maysL.) under nano TiO2spraying at various growth stages. Springer Plus, Vol. 2.

20:Mohammadi, R., R. Maali-Amiri and A. Abbasi, 2013.2nanoparticles on chickpea response to cold stress. Biol. Trace Element Res., 152: 403-410.

21:Singh, J. and B.K. Lee, 2016.2particles on the bioaccumulation of Cd in soybean plants (Glycine max): A possible mechanism for the removal of Cd from the contaminated soil. J. Environ. Manage., 170: 88-96.

22:Da Costa, M.V.J. and P.K. Sharma, 2015.Oryza sativa. Int. J. Rec. Scient. Res., 6: 2445-2451.

23:Tripathi, D.K., Shweta, S. Singh, S. Singh and R. Pandeyet al., 2017.

24:Tumburu, L., C.P. Andersen, P.T. Rygiewicz and J.R. Reichman, 2015.Arabidopsisgerminants. Environ. Toxicol. Chem., 34: 70-83.

25:Forsberg, E.M. and C. de Lauwere, 2013.

26:Ditta, A., M. Arshad and M. Ibrahim, 2015.

27:Chen, H. and R. Yada, 2011.

28:Parisi, C., M. Vigani and E.R. Cerezo, 2014.

29:Mahmoodzadeh, H., M. Nabavi and H. Kashefi, 2013.Brassica napus). J. Ornamental Hortic. Plants, 3: 25-32.

30:Khodakovskaya, M.V., K. de Silva, A.S. Biris, E. Dervishi and H. Villagarcia, 2012.

31:Ma, X., J. Geiser-Lee, Y. Deng and A. Kolmakov, 2010.

32:Jaberzadeh, A., P. Moaveni, H.R.T. Moghadam and H. Zahedi, 2013.

33:Yang, X., C. Cao, L. Erickson, K. Hohn, R. Maghirang and K. Klabunde, 2008.2-based photocatalysts by carbon and nitrogen doping. J. Catal., 260: 128-133.

34:Mishra, V., R.K. Mishra, A. Dikshit and A.C. Pandey, 2014.

35:Higashimoto, S., 2019.

36:Zheng, L., F. Hong, S. Lu and C. Liu, 2005.2on strength of naturally aged seeds and growth of spinach. Biol. Trace Elem. Res., 104: 83-91.

37:Feizi, H., P.R. Moghaddam, N. Shahtahmassebi and A. Fotovat, 2012.2) on wheat seed germination and seedling growth. Biol. Trace Element Res., 146: 101-106.

38:Servin, A.D., H. Castillo-Michel, J.A. Hernandez-Viezcas, B.C. Diaz, J.R. Peralta-Videa and J.L. Gardea-Torresdey, 2012.2nanoparticles in cucumber (Cucumis sativus) plants. Environ. Sci. Technol., 46: 7637-7643.

39:Feizi, H., M. Kamali, L. Jafari and P.R. Moghaddam, 2013.Foeniculum vulgareMill). Chemosphere, 91: 506-511.

40:Mahmoodzadeh, H. and R. Aghili, 2014.Triticum aestivumL.) seeds exposed to TiO2nanoparticles. J. Chem. Health Risks, 4: 29-36.

41:Haghighi, M. and J.A.T. da Silva, 2014.2on tomato, onion and radish seed germination. J. Crop Sci. Biotechnol., 17: 221-227.

42:Rezaei, F., P. Moaveni and H. Mozafari, 2015.2spraying on quantitative and qualitative yield of soybean (Glycine maxL.) at Shahr-e-Qods, Iran. Biol. Forum Intl. J. Biol. Forum, 7: 957-964.

43:Andersen, C.P., G. King, M. Plocher, M. Storm, L.R. Pokhrel, M.G. Johnson and P.T. Rygiewicz, 2016.

44:Gogos, A., J. Moll, F. Klingenfuss, M. van der Heijden and F. Irinet al., 2016.

45:Chaudhary, I.J. and D. Rathore, 2018.

46:Chaudhary, I.J. and D. Rathore, 2019.

47:Mohammadi, R., R. Maali-Amiri and N.L. Mantri, 2014.2nanoparticles on oxidative damage and antioxidant defense systems in chickpea seedlings during cold stress. Russian J. Plant Physiol., 61: 768-775.

48:Qi, M., Y. Liu and T. Li, 2013.2improve the photosynthesis of tomato leaves under mild heat stress. Biol. Trace Elem. Res., 156: 323-328.

49:Aghdam, M.T.B., H. Mohammadi and M. Ghorbanpour, 2016.Linum usitatissimum(Linaceae) under well-watered and drought stress conditions. Brazil. J. Bot., 39: 139-146.

50:Yang, W.W., A.J. Miao and L.Y. Yang, 2012.2+toxicity to a green algaChlamydomonas reinhardtiias influenced by its adsorption on TiO2engineered nanoparticles. PLoS One, Vol. 7, No. 3.

51:Paret, M.L., G.E. Vallad, D.R. Averett, J.B. Jones and S.M. Olson, 2013.2onXanthomonas perforansand control of bacterial spot of tomato. Phytopathology, 103: 228-236.

52:Li, J., M.S. Naeem, X. Wang, L. Liu, C. Chen, N. Ma and C. Zhang, 2015.2is not phytotoxic as revealed by the oilseed rape growth and photosynthetic apparatus ultra-structural response. PLoS One, Vol. 10, No. 12.

53:Ze, Y., C. Liu, L. Wang, M. Hong and F. Hong, 2011.2nanoparticles on the expression of light-harvesting complex II and photosynthesis of chloroplasts ofArabidopsis thaliana. Biol. Trace Elem. Res., 143: 1131-1141.

54:Hong, F., J. Zhou, C. Liu, F. Yang, C. Wu, L. Zheng and P. Yang, 2005.2on photochemical reaction of chloroplasts of spinach. Biol. Trace Elem. Res., 105: 269-279.

55:Hong, F., F. Yang, C. Liu, Q. Gao and Z. Wanet al., 2005.2on the chloroplast aging of spinach under light. Biol. Trace Elem. Res., 104: 249-260.

56:Lei, Z., S. Mingyu, W. Xiao, L. Chao and Q. Chunxianget al., 2008.

57:Lei, Z., S. Mingyu, L. Chao, C. Liang and H. Haoet al., 2007.2on photosynthesis of spinach chloroplasts under different light illumination. Biol. Trace Elem. Res., 119: 68-76.

58:Kiapour, H., P. Moaveni, D. Habibi and B. Sani, 2015.Ocimum basilicumL.). Int. J. Agron. Agric. Res., 6: 138-150.

59:Moaveni, P. and T. Kheiri, 2011.2nano particles affected on maize (Zea maysL.). Proceedings of the 2nd International Conference on Agricultural and Animal Science, IPCBEE, Volume 22, November 25-27, 2011, IACSIT Press, Singapore, pp: 160-163

60:Moaveni, P., A. Talebi, A. Farahani and K. Maroufi, 2011.2spraying on some yield components in barley (Hordem vulgareL.). Proceedings of the International Conference on Environmental and Agriculture Engineering IPCBEE, Volume 15, July 29-31, 2011, IACSIT Press, Jurong West, pp: 115-119

61:Raliya, R., P. Biswas and J.C. Tarafdar, 2015.2nanoparticle biosynthesis and its physiological effect on mung bean (Vigna radiateL.). Biotechnol. Rep., 5: 22-26.

62:Raliya, R., R. Nair, S. Chavalmane, W.N. Wang and P. Biswas, 2015.Solanum lycopersicumL.) plant. Metallomics, 7: 1584-1594.

63:Rafique, R., M. Arshad, M.F. Khokhar, I.A. Qazi, A. Hamza and N. Virk, 2015.

64:Gao, Y., W. Xiong, W. Ling and J. Xu, 2006.

65:Ma, X., R. Jian, P.R. Chang and J. Yu, 2008.

66:Cox, A., P. Venkatachalam, S. Sahi and N. Sharma, 2016.

67:Dolatabadi, A., B. Sani and P. Moaveni, 2015.Medicago scutellataL.). Cercetari Agronomice Moldova, 48: 53-61.

68:Burke, D.J., N. Pietrasiak, S.F. Situ, E.C. Abenojar and M. Porcheet al., 2015.

69:Song, G., Y. Gao, H. Wu, W. Hou, C. Zhang and H. Ma, 2012.2nanoparticles onLemna minor. Environ. Toxicol. Chem., 31: 2147-2152.

70:Kim, E., S.H. Kim, H.C. Kim, S.G. Lee, S.J. Lee and S.W. Jeong, 2011.

71:Clement, L., C. Hurel and N. Marmier, 2013.2nanoparticles to cladocerans, algae, rotifers and plants-effects of size and crystalline structure. Chemosphere, 90: 1083-1090.

72:Frazier, T.P., C.E. Burklew and B. Zhang, 2014.Nicotiana tabacum). Functional Integr. Genomics, 14: 75-83.

73:Song, U., H. Jun, B. Waldman, J. Roh, Y. Kim, J. Yi and E.J. Lee, 2013.Lycopersicon esculentum). Ecotoxicol. Environ. Saf., 93: 60-67.

74:Song, U., M. Shin, G. Lee, J. Roh, Y. Kim and E.J. Lee, 2013.2nanoparticle toxicity in three plant species. Biol. Trace Elem. Res., 155: 93-103.

75:Du, W., Y. Sun, R. Ji, J. Zhu, J. Wu and H. Guo, 2011.2and ZnO nanoparticles negatively affect wheat growth and soil enzyme activities in agricultural soil. J. Environ. Monit., 13: 822-828.

76:Gao, J., G. Xu, H. Qian, P. Liu, P. Zhao and Y. Hu, 2013.2on photosynthetic characteristics ofUlmus elongateseedlings. Environ. Pollut., 176: 63-70.

77:Castiglione, M.R., L. Giorgetti, C. Geri and R. Cremonini, 2011.2on seed germination, development and mitosis of root tip cells ofVicia narbonensisL. andZea maysL. J. Nanopart. Res., 13: 2443-2449.

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